238 Alleviation of neurogenic inflammation and reduction of skin aging signs, by blocking psychological stress signaling with a natural Cistus creticus extract

Psychological stress signaling affects skin via corticotropin releasing hormone (CRH) release. Through receptor CRH-R1, CRH activates inflammation mediators like NF-kB, IL1-β, IL6 and TNF-α in keratinocytes. Inflammation increases redness leads to extracellular matrix degradation, reduced elasticity / firmness, wrinkling. We show mitigation of these effects with an aqueous extract Cistus creticus, a Mediterranean shrub traditionally used as anti-inflammatory against some diseases. It contains the flavonoid myricitrin, tied IL6, – suggesting link extract’s effects. CRH-R1 blocking was tested functional assay recombinant CHO cells. An ex-vivo model measured master regulator NF-kB under stimulation (innumohistochemistry). A human keratinocyte culture downstream markers by ELISA. Finally, double-blind, placebo-controlled clinical trial, on highly stressed population, evaluated (microcirculation; redness; red spots; response SLS chemical insult) anti-aging (wrinkling). The effectively blocked indicating interruption neurogenic signaling. In model, decreased NF-kB. culture, expression TNF-α, negating CRH. trial showed significant (reduced microcirculation; increased resilience SLS, faster recovery; redness) These that mitigates interrupting cascade at its value for care applications.